David Eyre, PhD

Interests

We are studying the assembly of the extracellular matrix of skeletal tissues and molecular basis of various inborn and acquired skeletal disorders. The emphasis is on collagen structure and function, and current interests include: (1) mechanisms of collagen cross-linking in bone and cartilage (2) matrix assembly and degradation in cartilages (3) protein consequences of mutations causing chondrodysplasia syndromes (4) metabolic changes in cartilage in osteoarthritis (5) the extracellular matrix of the intervertebral disc and (6) molecular markers of bone and cartilage turnover.

Publications

1. Wu JJ, Weis MA, Kim LS, Carter BG, Eyre DR. Differences in Chain Usage and Cross-linking Specificities of Cartilage Type V/XI Collagen Isoforms with Age and Tissue. J Biol Chem. 2009 Feb 27;284(9):5539-45. Epub 2008 Dec 22.
2. Seegmiller RE, Bomsta BD, Bridgewater LC, Niederhauser CM, Montaño C, Sudweeks S, Eyre DR, Fernandes RJ. The heterozygous disproportionate micromelia (dmm) mouse: morphological changes in fetal cartilage precede postnatal dwarfism and compared with lethal homozygotes can explain the mild phenotype. J Histochem Cytochem. 2008 Nov;56(11):1003-11. Epub 2008 Aug 4.
3. Baldridge D, Schwarze U, Morello R, Lennington J, Bertin TK, Pace JM, Pepin MG, Weis M, Eyre DR, Walsh J, Lambert D, Green A, Robinson H, Michelson M, Houge G, Lindman C, Martin J, Ward J, Lemyre E, Mitchell JJ, Krakow D, Rimoin DL, Cohn DH, Byers PH, Lee B. CRTAP and LEPRE1 mutations in recessive osteogenesis imperfecta. Hum Mutat. 2008 Dec;29(12):1435-42.
4. Giunta C, Elçioglu NH, Albrecht B, Eich G, Chambaz C, Janecke AR, Yeowell H, Weis M, Eyre DR, Kraenzlin M, Steinmann B. Spondylocheiro dysplastic form of the Ehlers-Danlos syndrome–an autosomal-recessive entity caused by mutations in the zinc transporter gene SLC39A13. Am J Hum Genet. 2008 Jun;82(6):1290-305.
5. Eyre DR, Weis MA, Wu JJ. Advances in collagen cross-link analysis. Methods. 2008 May;45(1):65-74.
6. Fernandes RJ, Weis M, Scott MA, Seegmiller RE, Eyre DR. Collagen XI chain misassembly in cartilage of the chondrodysplasia (cho) mouse. Matrix Biol. 2007 Oct;26(8):597-603. Epub 2007 Jul 6.
7. Fernandes RJ, Harkey MA, Weis M, Askew JW, Eyre DR. The post-translational phenotype of collagen synthesized by SAOS-2 osteosarcoma cells. Bone. 2007 May;40(5):1343-51. Epub 2007 Jan 25.
8. Cabral WA, Chang W, Barnes AM, Weis M, Scott MA, Leikin S, Makareeva E, Kuznetsova NV, Rosenbaum KN, Tifft CJ, Bulas DI, Kozma C, Smith PA, Eyre DR, Marini JC. Prolyl 3-hydroxylase 1 deficiency causes a recessive metabolic bone disorder resembling lethal/severe osteogenesis imperfecta. Nat Genet. 2007 Mar;39(3):359-65. Epub 2007 Feb 4. Erratum in: Nat Genet. 2008 Jul;40(7):927.
9. Barnes AM, Chang W, Morello R, Cabral WA, Weis M, Eyre DR, Leikin S, Makareeva E, Kuznetsova N, Uveges TE, Ashok A, Flor AW, Mulvihill JJ, Wilson PL, Sundaram UT, Lee B, Marini JC. Deficiency of cartilage-associated protein in recessive lethal osteogenesis imperfecta. N Engl J Med. 2006 Dec 28;355(26):2757-64.
10. Morello R, Bertin TK, Chen Y, Hicks J, Tonachini L, Monticone M, Castagnola P, Rauch F, Glorieux FH, Vranka J, Bächinger HP, Pace JM, Schwarze U, Byers PH, Weis M, Fernandes RJ, Eyre DR, Yao Z, Boyce BF, Lee B. CRTAP is required for prolyl 3- hydroxylation and mutations cause recessive osteogenesis imperfecta. Cell. 2006 Oct 20;127(2):291-304.
11. Goto T, Matsui Y, Fernandes RJ, Hanson DA, Kubo T, Yukata K, Michigami T, Komori T, Fujita T, Yang L, Eyre DR, Yasui N. Sp1 family of transcription factors regulates the human alpha2 (XI) collagen gene (COL11A2) in Saos-2 osteoblastic cells. J Bone Miner Res. 2006 May;21(5):661-73.