UW School of Dentistry

Jeffrey

Jeffrey Scott McLean , PhD

Associate Professor, Periodontics

Office D-586, Lab D-558

Background

Dr. McLean received his PhD at the University of Southern California and his MSc at the University of Guelph in Canada. Dr. McLean’s research career at the Pacific Northwest National Laboratory, the J. Craig Venter Institute and now at the University of Washington School of Dentistry has been primarily devoted to developing innovative methodologies, tools and new genomic based approaches to study microbial interactions within oral biofilm communities. Currently, he is funded as a PI on multiple active NIH R01 grants that employ generation sequencing techniques such as single cell genomics and metatranscriptomics to characterize the microbial processes that lead to dental caries and maintain the health of the human oral microbiome. Within these projects, one goal is to isolate and capture genomes of oral pathogens and uncultivated novel oral phlya to gain a better understanding of these polymicrobial diseases and pathogen transmission routes.

McLean Research Lab Website

Awards and Recognition

  • Latornell Travel Award
  • National Science and Engineering Research Council (NSERC) Industrial Postgraduate Scholarship
  • Outstanding Performance Award, Scientific contribution to the D.O.E. Genomes to Life Microbial Cell Project
  • Outstanding Performance Award, Dedication and recognition for contributions toward the Microbial Cell Dynamics Laboratory
  • 2 National Laboratory On the Spot Awards for outstanding performance
  • Outstanding Performance Award, Scientific contribution to an ACS article nominated one of top 4 articles of 2007.

Recent Press Articles

Candidate Phylum TM7 and Periodontal Disease (Dec 2014-2015)

Novel genome of recovered from hospital (June 2013)

Single Cell genome of Periodontal Pathogen (2013)

Selected Recent Peer-reviewed Publications

*Corresponding Author #Co-first Author

  1. Guo, L.#, J. S. McLean#, Y. Yang, R. Eckert, C. W. Kaplan, P. Kyme, O. Sheikh, B. Varnum, R. Lux, W. Shi and X. He (2015). “Precision-guided antimicrobial peptide as a targeted modulator of human microbial ecology.” Proceedings of the National Academy of Sciences. published ahead of print June 1, 2015, doi:10.1073/pnas.1506207112Role: #Co-First Author. http://www.pnas.org/content/early/2015/05/27/1506207112.abstract OPEN ACCESS
  1. Anna Edlund, Youngik Yang, Shibu Yooseph, Adam P. Hall, Don D. Nguyen, Pieter C. Dorrestein, Karen E. Nelson, Xuesong He, Renate Lux, Wenyuan Shi, Jeffrey S. McLean* Meta-Omics Uncover Temporal Regulation of Pathways Across Oral Microbiome Genera During in vitro Sugar Metabolism. (2105)  International Society for Microbial Ecology Journal (ISME) http://www.nature.com/ismej/journal/vaop/ncurrent/full/ismej201572a.html OPEN ACCESS
  2. He, Xuesong#, McLean, Jeffrey S.#*(co-first author, co-corresponding author), Edlund, Anna, Yooseph, Shibu, Hall, Adam P., Liu, Su-Yang, Dorrestein, Pieter C., Esquenazi, Eduardo, Hunter, Ryan C., Cheng, Genhong, Nelson, Karen E., Lux, Renate, Shi, Wenyuan. (2015) Cultivation of a human-associated TM7 phylotype reveals a reduced genome and epibiotic parasitic lifestyle. Proceedings of the National Academy of Sciences 112(1):244-249. doi: 10.1073/pnas.1419038112. http://www.pnas.org/content/112/1/244.full OPEN ACCESS
  3. McLean JS* (2014) Advancements toward a systems level understanding of the human oral microbiome. Front Cell Infect Microbiol 4:98. PMCID: PMC4114298 http://journal.frontiersin.org/article/10.3389/fcimb.2014.00098/abstract
  4. Lasken RS & McLean JS (2014) Recent advances in genomic DNA sequencing of microbial species from single cells. Nat Rev Genet 15(9):577-584. PMID: 25091868 http://www.nature.com/nrg/journal/v15/n9/full/nrg3785.html
  5. McLean, JS.* and R. Lasken (2014). “Single cell genomics of bacterial pathogens: outlook for infectious disease research.” Genome Medicine 6(11): 108. http://genomemedicine.com/content/6/11/108
  6. Edlund, A., Yang, Y., Hall, A., Guo, L., Lux, R., He, X., Nelson, K., Nealson, K., Yooseph, S., Shi, W. & McLean, JS*. (2013) An in vitro biofilm model system maintaining a highly reproducible species and metabolic diversity approaching that of the human oral microbiome. Microbiome 1, 2 http://www.microbiomejournal.com/content/1/1/25
  7. McLean, JS*, M.-J. Lombardo, M. G. Ziegler, M. Novotny, J. Yee-Greenbaum, J. H. Badger, G. Tesler, S. Nurk, V. Lesin, D. Brami, A. P. Hall, A. Edlund, L. Z. Allen, S. Durkin, S. Reed, F. Torriani, K. H. Nealson, P. A. Pevzner, R. Friedman, J. C. Venter and R. S. Lasken (2013). “Genome of the pathogen Porphyromonas gingivalis recovered from a biofilm in a hospital sink using a high-throughput single-cell genomics platform.” Genome Research 23(5): 867-877. doi: 10.1101/gr.150433. PMCID: PMC3638142 http://genome.cshlp.org/content/23/5/867.long
  8. McLean, JS*, M.-J. Lombardo, J. H. Badger, A. Edlund, M. Novotny, J. Yee-Greenbaum, N. Vyahhi, A. P. Hall, Youngik Yang, C. L. Dupont, M. G. Ziegler, H. Chitsaz, A. E. Allen, S. Yooseph, G. Tesler, P. Pevzner, R. Friedman, K. H. Nealson, J. C. Venter and R. S. Lasken (2013). “Candidate phylum TM6 genome recovered from a hospital sink biofilm provides genomic insights into this uncultivated phylum.” PNAS. Jun 25;110(26):E2390-9. doi: 10.1073/pnas.1219809110 PMCID: PMC3696752 http://www.pnas.org/content/110/26/E2390.full
  9. McLean, JS *, S. J. Fansler, P. D. Majors, K. McAteer, L. Z. Allen, M. E. Shirtliff, R. Lux, W. Shi (2012) Identifying Low pH Active and Lactate-Utilizing Taxa within Oral Microbiome Communities from Healthy Children Using Stable Isotope Probing Techniques. PLoS ONE, 7 (3), e32219. PMCID:PMC3293899 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0032219
  10. McLean, JS*, O. N. Ona, and P. D. Majors. 2008. Correlated biofilm imaging, transport and metabolism measurements via combined nuclear magnetic resonance and confocal microscopy. ISME J 2:121 – 31. http://www.nature.com/ismej/journal/v2/n2/full/ismej2007107a.html

Additional publications

  1. He, X., McLean, J.S., Guo, L., Lux, R. & Shi, W.(2013). The social structure of a microbial community involved in colonization resistance. ISME J. Online 3 October 2013 doi:10.1038 /ismej.2013.172 PMCID: PMC3930314 http://www.nature.com/ismej/journal/v8/n3/full/ismej2013172a.html
  2. Guo, L., W. Hu, X. He, R. Lux, J. McLean and W. Shi (2013). “Investigating Acid Production by Streptococcus mutans with a Surface-Displayed pH-Sensitive Green Fluorescent Protein.” PLoS ONE 8(2): e57182. PMID: 23468929 PMCID: PMC3585301 http://www.plosone.org/article/authors/info%3Adoi%2F10.1371%2Fjournal.pone.0057182
  3. Allen LZ, Ishoey T, Novotny MA, McLean JS, Lasken RS, et al. (2011) Single Virus Genomics: A New Tool for Virus Discovery. PLoS ONE 6(3): e17722. doi:10.1371/journal.pone.0017722 PMID: 21436882 PMCID: PMC3059205 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0017722
  4. Tian, Y.; He, X.; Torralba, M.; Yooseph, S.; Nelson, K. E.; Lux, R.; McLean, J. S.; Yu, G.; Shi, W. (2010) Using DGGE profiling to develop a novel culture medium suitable for oral microbial communities. Mol Oral Microbiol, 25 (5), 357-67. PMCID: PMC2951289  http://onlinelibrary.wiley.com/doi/10.1111/j.2041-1014.2010.00585.x/full
  5. McLean, J. S*.; Wanger G, Gorby YA, Wainstein M, McQuaid J, Ishii SI, Bretschger O, Beyenal H, Nealson KH (2010) Quantification of Electron Transfer Rates to a Solid Phase Electron Acceptor through the Stages of Biofilm Formation from Single Cells to Multicellular Communities. Environ Sci Technol 44: 2721-2727. PMID: 20199066 http://pubs.acs.org/doi/abs/10.1021/es903043p McLean, Jeff EST single cell es903043p
  6. McLean, J. S., Pinchuk, G. E., Geydebrekht, O. V., Bilskis, C. L., Zakrajsek, B. A., Hill, E. A., Saffarini, D. A., Romine, M. F., Gorby, Y. A., Fredrickson, J. K., and Beliaev, A. S., 2008. Oxygen-dependent autoaggregation in Shewanella oneidensis MR-1. Environ. Microbiol. Jul;10(7):1861-76. Epub 2008 Apr 10 doi:10.1111/j.1462-2920.2008.01608.x PMID: 18412550 http://onlinelibrary.wiley.com/doi/10.1111/j.1462-2920.2008.01608.x/abstract PDF
  7. McLean, J. S., P. D. Majors, C. L. Reardon, C.L. Bilskis, S.B. Reed, M. F. Romine and J. K. Fredrickson. (2007). “Investigations of structure and metabolism within Shewanella oneidensis MR-1 biofilms.” Journal of Microbiological Methods. 74: 47-56. 10.1016/j.mimet.2008.02.015
  8. Pinchuk, G. E., C. Ammons, D. E. Culley, S. M. Li, J. S. McLean, M. F. Romine, K. H. Nealson, J. K. Fredrickson, and A. S. Beliaev. ( 2007). Utilization of DNA as a Sole Source of Phosphorus, Carbon, and Energy by Shewanella: Ecological and Physiological Implications for Dissimilatory Metal Reduction. Appl Environ Microbiol. 74(4):1198-208.
  9. Gorby YA, S Yanina, JS McLean, KM Rosso, DM Moyles, A Dohnalkova, TJ Beveridge, I Chang, BH Kim, KS Kim, DE Culley, SB Reed, MF Romine, D Saffarini, EA Hill, L Shi, DA Elias, DW Kennedy, GE Pinchuk, K Watanabe, S Ishii, B Logan, KH Nealson, and JK Fredrickson. (2006). “Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms.” Proceedings of the National Academy of Sciences of the United States of America 103(30):11358-11363.
  10. McLean JS, Beveridge TJ. (2001). Chromate reduction by a pseudomonad isolated from a site contaminated with chromated copper arsenate. Appl Environ Microbiol. Mar; 67(3):1076-84.
  11. McLean JS, Beveridge TJ, Phipps D. (2000). Isolation and characterization of a chromium-reducing bacterium from a chromated copper arsenate-contaminated site. Environ Microbiol. Dec; 2(6):611-9.

Book Chapters

  1. Majors, P.D. and J.S. McLean. (2009). “Dynamic Metabolism Studies of Live Bacterial Films.” in Magnetic Resonance Microscopy. S. L. Codd and J. D. Seymour. Weinheim, Wiley-VCH. http://dx.doi.org/10.1002/9783527626052 NLM Unique ID: 101511650
  2. McLean J.S., Lee JU, and Beveridge T.J.( 2001). Interactions of Bacteria and Environmental Metals, Fine-Grained Mineral Development and Bioremediation Strategies. In Interactions Between Soil Particles and Microorganisms and Their Impact on the Terrestrial Environment. (Ed. PM Huang) IUPAC Series on Analytical and Physical Chemistry of Environmental Systems. John Wiley and Sons. Ltd., UK.
  3. Mclean, J., T. Beveridge and D. Phipps. (1999) Chromate removal from contaminated groundwater using indigenous bacteria. In Bioremediation of Metals and Inorganic Compounds (Eds. A Leeson and B.C. Alleman). Proceedings of the In Situ and On Site Bioremediation . Fifth International Symposium. April 19-22, , San Diego. California, Battelle Press