Timothy Rose, PhD
Adjunct Professor, Oral Health Sciences
Research in the Rose Lab is focused on herpesviruses implicated in cellular transformation and tumor induction, and in the study of host and viral proteins that mediate these effects. In particular, we are studying the viral etiology of Kaposi’s sarcoma (KS) and other AIDS-related malignancies with regards to the interactions between viruses (retroviruses and herpesviruses) and cytokines in virus activation and tumor induction. Our research focuses on the Kaposi’s sarcoma-associated herpesvirus (KSHV) and its homologs in non-human primates. We discovered the macaque homolog of KSHV and are studying its association with a Kaposi’s sarcoma-like malignancy in macaques, called retroperitoneal fibromatosis (RF), which like AIDS-KS is associated with a retrovirus infection. RF occurs in conjunction with simian AIDS (SAIDS) caused by infection with simian retrovirus 2 (SRV2) or SIV, the simian homolog of HIV. We have identified a second lineage of KSHV-like rhadinoviruses in macaques and are studying its role in RF and other macaque tumors. Ongoing projects include the cloning and sequence of the genome of the new macaque herpesvirus, the identification and characterization of cellular receptors mediating infection by KSHV, the comparative analysis of KSHV and its simian homologs and their role in tumor induction in association with HIV induced immunosuppression, the characterization of latency and the activating switch to herpesvirus replication, and the development of diagnostic tests for novel viruses.
PABIO 536 – Bioinformatics and Gene Sequence Analysis
- Yilmaz O, Yao L, Maeda K, Rose TM, Lewis EL, Duman M, Lamont RJ, Ojcius DM (2008). ATP scavenging by the intracellular pathogen Porphyromonas gingivalis inhibits P2X7-mediated host-cell apoptosis. Cell Microbiol 10(4):863-875.
- Garrigues HJ, Rubinchikova YE, Dipersio CM, Rose TM (2008). Integrin alphaVbeta3 Binds to the RGD motif of glycoprotein B of Kaposi’s sarcoma-associated herpesvirus and functions as an RGD-dependent entry receptor. J Virol 82(3):1570-1580.
- Bruce AG, Bakke AM, Bielefeldt-Ohmann H, Ryan JT, Thouless ME, Tsai CC, Rose TM (2006). High levels of retroperitoneal fibromatosis (RF)-associated herpesvirus in RF lesions in macaques are associated with ORF73 LANA expression in spindleoid tumour cells. J Gen Virol 87(Pt 12):3529-3538.
- Burnside KL, Ryan JT, Bielefeldt-Ohmann H, Gregory Bruce A, Thouless ME, Tsai CC, Rose TM (2006). RFHVMn ORF73 is structurally related to the KSHV ORF73 latency-associated nuclear antigen (LANA) and is expressed in retroperitoneal fibromatosis (RF) tumor cells. Virology 354(1):103-115.
- Philipp-Staheli J, Marquardt T, Thouless ME, Bruce AG, Grant RF, Tsai CC, Rose TM (2006). Genetic variability of the envelope gene of Type D simian retrovirus-2 (SRV-2) subtypes associated with SAIDS-related retroperitoneal fibromatosis in different macaque species. Virol J 3:11.
- Philipp-Staheli, J., Marquardt, T., Thouless, M.E., Bruce, A.G., Grant, R.F., Tsai, C.-C., and Rose, T.M. (2006) Genetic variability of the envelope gene of Type D simian retrovirus-2 (SRV-2) subtypes associated with SAIDS-related retroperitoneal fibromatosis in different macaque species. Virol. J. (In Press)
- Burton, M., Rose, T.M., Færgeman, N.J. and Knudsen, J. (2005) Structure and evolution of the ACBP gene family. Biochem J. 392, 299-307
- Rose, T.M. (2005) Consensus-Degenerate Hybrid Oligonucleotide Primer (CODEHOP)-mediated PCR – A powerful technique for the identification and characterization of viral genomes. Virology Journal 2, 20
- Bielefeldt-Ohmann, H., Barouch, D.H., Bakke, A.M., Bruce, A.G., Durning, M., Grant, R., Letvin, N.L., Ryan, J.T., Schmidt, A., Thouless, M.E., and Rose, T.M. (2005) Intestinal Stromal Tumors in a SIV-infected, SRV-2 negative, rhesus macaque (Macaca mulatta) Veterinary Pathology 42: 391-396.
- Bruce, A.G., A.M. Bakke, and T.M. Rose. (2005) Development of a real-time Q-PCR assay for the detection of rhadinovirus-2 lineage-specific viruses in macaques and baboons. Virology Journal. 2, 2.
- Rose, T.M., Henikoff, J. G. and Henikoff, S. (2003) CODEHOP (Consensus-Degenerate Hybrid Oligonucleotide Primer) PCR Primer Design. Nucleic Acids Res. 31:3763-6
- Rose, T.M., Schultz, E.R., Ryan, J.T., Raden, B. and Tsai. C.-C. (2003) Analysis of 4.3 kb of the divergent locus-B (DL-B) of the macaque RFHV reveals a close similarity to the Kaposi’s sarcoma-associated herpesvirus (KSHV) in gene sequence and genome organization. J. Virology, 77, 5084-5097.
- Hermouet, S., Sutton, C.A., Rose, T.M., Greenblatt, R.J., Corre, I., Garand, R., Neves A.M., Bataille, R., Casey, J.W. (2003) Qualitative and quantitative analysis of human herpesviruses in chronic and acute B-cell lymphocytic leukemia and in multiple myeloma. Leukemia 17:185-95.
- Xie, H., Cook, G.S., Costerton, J.W., Bruce, A.G., Rose, T.M., Lamont, R.J. (2000) Intergeneric Communication in Dental Plaque Biofilms. J. Bacteriology 182, 7067-7069.
- Schultz, E.R., Rankin, G.W. Jr., Blanc, M.-P., Raden, B.W., Tsai, C.-C. and Rose, T.M. (2000) Characterization of two divergent lineages of macaque rhadinoviruses related to Kaposi’s sarcoma-associated herpesvirus. J. Virol. 74, 4919-4928.